Dear users,
I am working on monoclonal antibody exhibiting target mediated drug disposition
and trying to fit a quasi-equilibrium model described by Mager and Krzyzanski
[Pharm Res 22(10):1598]. In the paper, the quasi-equilibrium was fitted to
total drug conc. and total target conc. We observed total drug conc., total
target conc., and free target conc. I am trying to fit the model to not only
total drug conc. and total target conc. but also free target conc.
simutaneously. But I did not figure out how I can incorporate free target conc.
in the model using KD instead of Kon and Koff. Could anybody please give me
some suggestion or help? Thank you.
Best,
Xiaofeng
Modeling targeted mediated drug disposition using Quasi-equilibrium model
Xiaofeng
you can use:
Rfree= Rtot *KD/(KD+Cfree)
Leonid
--------------------------------------
Leonid Gibiansky, Ph.D.
President, QuantPharm LLC
web: www.quantpharm.com
e-mail: LGibiansky at quantpharm.com
tel: (301) 767 5566
Quoted reply history
On 7/6/2012 11:40 AM, Wang, Xiaofeng wrote:
> Dear users,
>
> I am working on monoclonal antibody exhibiting target mediated drug
> dispositionand trying to fit a quasi-equilibriummodel described by
> Magerand Krzyzanski[PharmRes 22(10):1598]. In the paper, the
> quasi-equilibriumwas fitted to total drug conc. and total target conc.
> We observed total drug conc., total target conc., and free target conc.
> I am trying to fit the model to not only total drug conc. and total
> target conc. but also free target conc. simutaneously. But I did not
> figure out how I can incorporate free target conc. in the model using
> KDinstead of Konand Koff. Could anybody please give me some suggestion
> or help? Thank you.
>
> Best,
>
> Xiaofeng
>
> No virus found in this message.
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