question about sampling time

From: Winnie Seto Date: October 27, 2013 technical Source: mail-archive.com
Hello NONMEM users and Dr. Holford I am a novice when it comes to pop PK simulations- Our group is planning on a prospective once daily aminoglycoside pharmacokinetic study in patients with proposed serial time levels post dose with 30min, 2hr, 4hr, 12hr and 24hr post dose drug levels. We will have only a small sample size (less than 50 patients) and some patients may not be able to have samples at all these timepoints from logistical reasons. I would really appreciate some advice on the following: 1. Is there a number of minimum patients that I need for each sampling time point for pop pk analysis? 2. For each time point - is it better to have some variability within the sampling time window e.g. 10-12hr with a few patients with samples distributed at 10, 11 or 12 hour mark? 3. I have learnt only the basics with NONMEM; I have used WIN NONLIN in the past - recently I have added their WIN-NLME pop PK software to my subscriptions - should I use both NONMEM and NLME to run results ? would there be differences? I suspected that NLME may be easier for novice and students to catch up during short-student rotations to run simulations then to learn NONMEM on a 4 week rotation. Any opinions? Thank you in advance for your help. Winnie Winnie Seto, BScPhm, PharmD, MSc, ACPR, R.Ph. Therapeutic Drug Monitoring Coordinator & Critical Care Unit Pharmacist Clinical Manager Department of Pharmacy The Hospital for Sick Children 555 University Avenue Toronto, Ontario Canada M5G 1X8 phone: 416-813-6236 fax: 416-813-5880 Confidentiality Notice: This is a medical communication. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain privileged and confidential information. Any unauthorized review, use, copying, saving, re-transmission, disclosure or distribution is strictly prohibited. If you are not the intended recipient, please contact the author by reply email and destroy all original and copies of this email message. ________________________________
Oct 27, 2013 Winnie Seto question about sampling time
Oct 27, 2013 Nick Holford Re: question about sampling time
Oct 28, 2013 Winnie Seto Re: Re: question about sampling time