RE: protein binding

From: "Ekaterina Gibiansky" gibianskye@guilfordpharm.com Subject: RE:[NMusers] protein binding Date: Thu, June 24, 2004 1:30 pm Anthe, not being at the course, I do not know what you are trying to accomplish. But from your data and control stream it seems that you observe only total concentrations. If so, I do not get why you need all these compartments and complexities, you only need one for total concentration: DADT(1)=-K10*A(1)*(1-BMAX*/(KD+A(1)) C1=A(1)/V ; total conc and you need to bind Bmax/KD <1 (i.e. reparameterize to have Bmax/KD, and KD to be independent variables). Also, with the control stream you have now you do not fix parameter values for simulation. NONMEM seems to take your upper boundaries. Is it what you intended? Looking at your conversation with Leonid, it seems you want to incorporate association/dissociation rates in your model. But this is not done with the contol stream you have: with algebraic equations your binding/unbinding processes are instant. Katya _______________________________________________________