Antwort: Re: enzyme auto inhibition PK model

1 messages 1 people Latest: Jun 06, 2008

Antwort: Re: enzyme auto inhibition PK model

From: Nele . Plock Date: June 06, 2008 technical
Dear Yuan, it is good to hear that the article was useful. The model was completely identifiable the way I wrote it. The reason for this was that I had very dense concentration-time data in the same individual after single as well as repeated dosing, and just by visual inspection of the profiles you could see the change in the terminal slope. There was not good reason to assume that this was due to a change in volume of distribution, so it had to be clearance. The inhibition compartment is not a physiological compartment but an empiric approach to describe the changes. However, the physiologic explanation for the inhibition is given in the article. The inhibition compartment is just an ordinary effect compartment. A2 corresponds to the amount of drug in the central compartment, so A2/V2 is a concentration. And if concentration is coming in, then concentration is also going out (i.e. A4 is a concentration). If you have any questions, please feel free to give me a call. Also, if you want to read more, you might want to have a look at my dissertation: http://sundoc.bibliothek.uni-halle.de/diss-online/07/07H049/prom.pdf or as a book: Dr. Plock, Nele: Pharmacokinetics of Antiinfectives in the Treatment of Serious Grampositive Infections - Linezolid and Vancomycin (ISBN 978-3-86553-218-3, Wiku, Germany, 2007) Best regards Nele _________________________ Dr. Nele Plock Bayer Schering Pharma AG Drug Metabolism & Pharmacokinetics Development Pharmacokinetics Scientific Expert Development Pharmacokinetics D- 13342 Berlin Phone : +49-30-468 15146 Fax: +49-30-468 95146 [EMAIL PROTECTED] http://www.bayerscheringpharma.de Vorstand: Arthur J. Higgins, Vorsitzender | Werner Baumann, Andreas Busch, Ulrich Köstlin, Kemal Malik, Gunnar Riemann Vorsitzender des Aufsichtsrats: Werner Wenning Sitz der Gesellschaft: Berlin | Eintragung: Amtsgericht Charlottenburg 93 HRB 283 "Yuyan Jin" <[EMAIL PROTECTED]> Gesendet von: [EMAIL PROTECTED] 06.06.2008 05:57 An "Murad Melhem" <[EMAIL PROTECTED]> Kopie [email protected] Thema Re: [NMusers] enzyme auto inhibition PK model Thanks Murad, I read the article you suggested tonight. this is the first paper I read so far modeled enzyme auto-inhibition and it is really useful. it is also nice to see that the article differentiate the inhibitable CL and non-inhibitable Cl. but I am wondering if these two CL could be accurately estimated without corresponding metabolites data? also, I still could not figure out what the inhibition component refers to physiologically? and why def equation for the auto-inhibition comparment was written as: dA4/dt=Kic*( A2 / V2-A4) isn't unit of A2 and A4 the same? Can anyone give me some hint? Sincerely, Yuyan
Quoted reply history
On 6/5/08, Murad Melhem <[EMAIL PROTECTED]> wrote: Hi Yuyan, Mechanistic models incorporating an auto-inhibition component would be more desirable. However, you may be able to describe the PK data of your compound empirically by adding an additional inhibition compartment. Depending on the concentration in this empirical compartment, systemic CL from the central compartment is inhibited. Over time, you can allow the CL to take values between 0 and 100% of the original value estimated for the time of the first administration. A good reference for such an empirical approach is: Plock N, Buerger C, Joukhadar C, Kljucar S, Kloft C. Does linezolid inhibit its own metabolism? Population pharmacokinetics as a tool to explain the observed nonlinearity in both healthy volunteers and septic patients. Drug Metab Dispos. 2007 Oct;35(10):1816-23. I hope this helps. Murad Melhem, PhD Assistant Director PK/PD Cognigen Corporation Buffalo, NY On Thu, Jun 5, 2008 at 5:06 PM, Yuyan Jin <[EMAIL PROTECTED]> wrote: Hi Dear Nonmem user, I am trying to model the kinetics of a compound that exhibits enzyme auto-inhibition.I have found some previously suggested literature by nonmem users, but all of them were auto-induction cases. Does anyone know any enzyme auto-inhibition PK model paper published ? Thanks so much! Sincerely, Yuyan Jin Graduate student University of Pittsburgh -- Yuyan Jin