Single Trough Levels

Dear group, I am working on a retrospectively collected dataset from hospital based subjects. I have only one trough measured for each subject after IV infusion. My understanding is that we need at least two samples in the same subject to estimate BSV and ISV. We would like to develop a population PK model with the data to explore the potential optimization of the therapy using the model simulations. I appreciate if you can direct me to the proper references and any ideas on modeling. Also, I appreciate your comments on the parametric vs nonparametric approaches in such scenarios where one performs better than other, if at all. Ayyappa Chaturvedula, PhD Visiting Assistant Research Professor, College of Pharmacy and Health Sciences, Mercer University, 3001 Mercer University Drive, Atlanta, GA 30341 6785476168 (O)