Modeling biomarker data below the LOQ

From: Sameer Doshi Date: November 18, 2009 technical Source: mail-archive.com
Hello, We are attempting to model suppression of a biomarker, where a number of samples (40-60%) are below the quantification limit of the assay and where 2 different assays (with different quantification limits) were used. We are trying to model these BQL data using the M3 and M4 methods proposed by Ahn et al (2008). I would like to know if anyone has any comments or experience implementing the M3 or M4 methods for biomarker data, where levels are observed at baseline, are supressed below the LOQ for a given duration, and then return to baseline. Also please advise if there are other methods to try and incorporate these BQL data into the model. I have included the relevant pieces of the control file (for both M3 and M4) and data from a single subject. Thanks for your review/suggestions. Sameer DATA: #ID TIME AMT DV CMT EVID TYPE ASSY 1 0 0 65.71 0 0 0 1 1 0 120 0 3 1 0 1 1 168 0 10 0 0 1 1 1 336 0 10 0 0 1 1 1 336 120 0 3 1 0 1 1 504 0 12.21 0 0 0 1 1 672 120 0 3 1 0 1 1 1008 0 10 0 0 1 1 1 1008 120 0 3 1 0 1 1 1344 0 10 0 0 1 1 1 1344 120 0 3 1 0 1 1 1680 0 10 0 0 1 1 1 1680 120 0 3 1 0 1 1 2016 0 10 0 0 0 1 1 2352 0 25.64 0 0 0 1 1 2688 0 59.48 0 0 0 1 MODEL M3: $DATA data.csv IGNORE=# $SUB ADVAN8 TRANS1 TOL=6 $MODEL COMP(central) COMP(peri) COMP(depot,DEFDOSE) COMP(effect) $DES DADT(1) = KA*A(3) - K10*A(1) - K12*A(1) + K21*A(2) DADT(2) = K12*A(1) - K21*A(2) DADT(3) = -KA*A(3) CONC = A(1)/V1 DADT(4) = KEO*(CONC-A(4)) $ERROR CALLFL = 0 LOQ1=10 LOQ2=20 EFF = BL* (1 - IMAX*A(4)**HILL/ (IC50**HILL+A(4)**HILL)) IPRED=EFF SIGA=THETA(7) STD=SIGA IF(TYPE.EQ.0) THEN ; GREATER THAN LOQ F_FLAG=0 Y=IPRED+SIGA*EPS(1) IRES =DV-IPRED IWRES=IRES/STD ENDIF IF(TYPE.EQ.1.AND.ASSY.EQ.1) THEN ; BELOW LOQ1 DUM1=(LOQ1-IPRED)/STD CUM1=PHI(DUM1) F_FLAG=1 Y=CUM1 IRES = 0 IWRES=0 ENDIF IF(TYPE.EQ.1.AND.ASSY.EQ.2) THEN ; BELOW LOQ2 DUM2=(LOQ2-IPRED)/STD CUM2=PHI(DUM2) F_FLAG=1 Y=CUM2 IRES = 0 IWRES=0 ENDIF $SIGMA 1 FIX $ESTIMATION MAXEVAL=9990 NOABORT SIGDIG=3 METHOD=1 INTER LAPLACIAN POSTHOC PRINT=2 SLOW NUMERICAL $COVARIANCE PRINT=E SLOW MODEL M4: $DATA data.csv IGNORE=# $SUB ADVAN8 TRANS1 TOL=6 $MODEL COMP(central) COMP(peri) COMP(depot,DEFDOSE) COMP(effect) $DES DADT(1) = KA*A(3) - K10*A(1) - K12*A(1) + K21*A(2) DADT(2) = K12*A(1) - K21*A(2) DADT(3) = -KA*A(3) CONC = A(1)/V1DADT(4) = KEO*(CONC-A(4)) $ERROR CALLFL = 0 LOQ1=10 LOQ2=20 EFF = BL* (1 - IMX*A(4)**HILL/ (IC50**HILL+A(4)**HILL)) IPRED=EFF SIGA=THETA(7) STD=SIGA IF(TYPE.EQ.0) THEN ; GREATER THAN LOQ F_FLAG=0 YLO=0 Y=IPRED+SIGA*EPS(1) IRES =DV-IPRED IWRES=IRES/STD ENDIF IF(TYPE.EQ.1.AND.ASSY.EQ.1) THEN DUM1=(LOQ1-IPRED)/STD CUM1=PHI(DUM1) DUM0=-IPRED/STD CUMD0=PHI(DUM0) CCUMD1=(CUM1-CUMD0)/(1-CUMD0) F_FLAG=1 Y=CCUMD1 IRES = 0 IWRES=0 ENDIF IF(TYPE.EQ.1.AND.ASSY.EQ.2) THEN DUM2=(LOQ2-IPRED)/STD CUM2=PHI(DUM2) DUM0=-IPRED/STD CUMD0=PHI(DUM0) CCUMD2=(CUM2-CUMD0)/(1-CUMD0) F_FLAG=1 Y=CCUMD2 IRES = 0 IWRES=0 ENDIF $SIGMA 1 FIX $ESTIMATION MAXEVAL=9990 NOABORT SIGDIG=3 METHOD=1 INTER LAPLACIAN POSTHOC PRINT=2 SLOW NUMERICAL $COVARIANCE PRINT=E SLOW Sameer Doshi Pharmacokinetics and Drug Metabolism, Amgen Inc. (805) 447-6941
Nov 18, 2009 Sameer Doshi Modeling biomarker data below the LOQ
Nov 18, 2009 Leonid Gibiansky Re: Modeling biomarker data below the LOQ
Nov 18, 2009 Mats Karlsson RE: Modeling biomarker data below the LOQ
Nov 19, 2009 Leonid Gibiansky Re: Modeling biomarker data below the LOQ
Nov 20, 2009 Leonid Gibiansky Re: Modeling biomarker data below the LOQ
Nov 20, 2009 Martin Bergstrand RE: Modeling biomarker data below the LOQ
Nov 20, 2009 Jurgen Bulitta RE: Modeling biomarker data below the LOQ